Instruction
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Fill out the requested information in the forms as clear as possible.」
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Attach an image of the electrophoresis gel
Please indicate the amount of DNA sample loaded on the gel and the type of DNA marker used. Concentration and amount of DNA and Primer required：
- Sequencing of single/double strand DNA and PCR product
- DNA and primer must be dissolved in ddH2O, and the OD260/OD280 ratio must be greater than 1.8.
- PCR products must be purified before submission.
- If PCR products contain multiple bands, DNA for the correct band must be purified before submission.
- The amount of DNA required for submission: DNA=10 μl / reaction, primer= 5 μl / reaction. The detailed concentrations are as follows：
Concentration Table
Template Type Template (ng/μl) Primer (ng/μl) Standard Primer Provided by BTC PCR product:
< 200 bp
200 - 500 bp
500 - 1000 bp
1000 - 2000 bp
> 2000 bp
(ng/ul)
10 (ng/ul)
20 (ng/ul)
40 (ng/ul)
80 (ng/ul)
100 (ng/ul)
5 (pmol/ul) T7： 5' TAATACGACTCACTATAGGG 3'
T3： 5' ATTAACCCTCACTAAAGGGA 3'
T7 terminator： 5' GCTAGTTATTGCTCAGCGGT3'
SP6： 5' ATTTAGGTGACACTATAG 3'
M13-forward： 5' TGTAAAACGACGGCCAGT 3'
M13-reverse： 5' TCACACAGGAAACAGCTATGAC 3'
Other primer: Please bring your ownSingle-strended DNA 100 (ng/ul) 5 (pmol/ul) Double-strended DNA
(4-15 kb)200 (ng/ul) 5 (pmol/ul) Large DNA
(plasmids over ~ 20 kb, cosmids, lambda clones)500 (ng/ul) 30 (pmol/ul) Large DNA
(P1 colnes, BACs)1000 (ng/ul) 30 (pmol/ul)
